柔嫩艾美耳球虫多肽:N-乙酰氨基半乳糖转移酶2的基因表达及转录动态分析

doi:10.11843/j.issn.0366-6964.2017.11.019

Abstract

Abstract:

O-linked glycosylation is a form of protein posttranslational modifications that occurs in the Golgi apparatus of eukaryotes, and archaea as well as bacteria. Polypeptide:N-acetylgalactosaminyltransferase 2 (ppGalNAc-T2, EC 2.4.1.41) is the first key enzyme in the pathway of O-glycan biosynthesis. Whole genome sequencing of Eimeria spp has ever revealed that these protozoa possess the pathways for O-glycan synthesis and O-linked glycosylation of proteins. For exploring the profiles of glycosylation in the global proteome of Eimeria tenella during developmental cycle, and the potential druggable target of EtppGalNAc-T2, we have cloned and recombinantly expressed the EtppGalNAc-T2 gene according to its predicted coding sequence deposited in EuPathDB, and evaluated its transcriptional profiling in different stages of life cycle using qRT-PCR method. The results showed that the ORF of EtppGalNAc-T2 is 1 983 bp in full length, and can be expressed in E. coli Transetta (DE3) with recombinant vector EtppGalNAc-T2-pGEX-6p-1. qRT-PCR results showed that the transcriptions of EtppGalNAc-T2 are significantly varied among different developmental stages, that is, the highest in E. tenella sporozoite, and nearly no-detectable transcription in sporulated occyst. Briefly, these results confirmed O-glycan synthesis process, and may play roles in the regulation of developmental conversion in E. tenella.

CLC Number:

S852.723

LIU Xiao-li, GONG Zhen-xing, MA Xue-ting, QU Zi-gang, HAN Zheng-lan, LIU Bao-hong, YUAN Ru-yi, YANG Xiao-pu, CAI Jian-ping. Expression Profiling of Polypeptide:N-acetylgalactosaminyltransferase T2 during Eimeria tenella Developmental Cycle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2173-2180.

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Expression Profiling of Polypeptide:N-acetylgalactosaminyltransferase T2 during Eimeria tenella Developmental Cycle

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